Plasmid

Codon Optimisation: A Hidden Process Behind IndieBB

I’ve posted twice recently after a prolonged blogging absence, and here I am again. Perhaps I should always be running a crowdfunding campaign, so that I have a stake in blogging; I’d be far more prolific! I’m too principled (or, to some “dogmatic”) to include advertisement on this or any blog, and flattr revenue is far too thin to encourage more than the occasional post otherwise. In any case, today I’d like to share something with you all that I’ve been meaning to write up for ages anyway, but which becomes especially relevant in light of my IndieBB DIYbio/Biohacking/Teaching plasmid design project.

Dear all, my DIYbio plasmid has arrived

Some time ago I placed an order for a labour of love of mine: IndieBB. It’s a plasmid I designed to make cloning in Bacillus subtilis easier, faster and more reliable. Crucially, it’s also supposed to make the whole process antibiotic-free and DIYbio friendly. It’s going to be the flagship product of Indie Biotech if it works, and if it sells well enough I’m planning to offer additional cassettes that extend and enhance the plasmid, allowing customers to start performing practical synthetic biology with a minimal lab setup requirement.